Recent Publications

Nomura M, Kuroda T, Ohta M, Kusano K, Minamijima Y, Nagata S.
Pharmacokinetics of Salbutamol in Thoroughbred Horses After a Single Intravenous or Inhaled Administration.
J Vet Pharmacol Ther, 2025, 48(2), 76-85.

Shimizu Y, Sugai-Bannai M, Saito K, Hirano-Kodaira M, Leung GN.
Identification and differentiation of somapacitan, a long-acting growth hormone derivative, and recombinant human growth hormone in equine plasma by LC-HRMS for doping control purpose.
Growth Hormone & IGF Research, 2025, 80, 101628.

Kuroda T, Minamijima Y, Niwa H, Mita H, Tamura N, Fukuda K, Toutain PL, Ohta M.
Pharmacokinetics/pharmacodynamics cut-off determination for fosfomycin using Monte Carlo simulation in healthy horses.
J Vet Med Sci, 2024, 86(4), 413-420.

Mita H, Kuroda T, Minamijima Y, Tamura N, Ohta M.
Concentration of Marbofloxacin in equine subcutaneous tissue fluid after subcutaneous administration in encapsulated microparticles.
J Equine Vet Sci, 2024, 141, 105148.

Kuroda T, Minamijima Y, Kinman CK, Takahashi Y, Ebisuda Y, Inoue K, Ishikawa H, Mita H, Tamura N, Nukada T, Toutain PL, Ohta M.
Rational quinidine dosage regimen for atrial fibrillation in Thoroughbred racehorses based on population pharmacokinetics.
Front Vet Sci, 2024, 11, 1454342.

Uchida T, Kisugi T, Ishii H, Yamada M, Kinoshita K, Leung GN.
Broad-spectrum and sensitive screening of more than 1000 compounds in equine urine using liquid chromatography/highresolution mass spectrometry
Rapid Commun Mass Spectrom, 2024, 38, e9856.

Ohnuma K, Hirano-Kodaira M, Bannai M, Shimizu Y, Yamada M, Kinoshita K, Leung GN, Ishii H.
A Broad-Spectrum Peptide Screening Method Using an Optimized Solid-Phase Extraction and Liquid Chromatography–High-Field Asymmetric Ion Mobility Spectrometry–Mass Spectrometry for Doping Control in Equine Urine.
Anal Methods, 2024, 16, 8250-8267.

Ishii H, Shigematsu R, Takemoto S, Ishikawa Y, Mizobe F, Nomura M, Arima D, Kunii H, Yuasa R, Yamanaka T, Tanabe S, Nagata S, Yamada M, Leung GN.
Quantification of osilodrostat in horse urine using LC/ESI-HRMS to establish an elimination profile for doping control.
Bioanalysis, 2024, 16(17-18), 947-958.

Ishii H, Shigematsu R, Takemoto S, Ishikawa Y, Mizobe F, Nomura M, Arima D, Kunii H, Yuasa R, Yamanaka T, Tanabe S, Nagata S, Yamada M, Leung GN.
Metabolic Pathway of Osilodrostat in Equine Urine Established through High-resolution Mass Spectrometric Characterization for Doping Control.
Current Drug Metabolism, 2024, 25(7), 489-504.

Minamijima Y, Kuroda T, Kamiya T, Sone Y, Wakuno A, Ito H, Nomura M, Leung GN, Kinoshita K, Yamada M.
Evaluation of plasma and urine pharmacokinetics of tranexamic acid for equine medication control.
J Vet Pharmacol Ther, 2024, 47(1), 21-27.

Kuroda T, Minamijima Y, Niwa H, Mita H, Tamura N, Fukuda K, Ohta M.
Pharmacokinetic/pharmacodynamic analysis of cephalothin after intramuscular administration in Thoroughbred horses.
Journal of Equine Science, 2023, 34(4), 111-114.

Lallemand EA, Bousquet-Mélou A, Chapuis L, Davis J, Ferran AA, Kukanich B, Kuroda T, Lacroix MZ, Minamijima Y, Olsén L, Pelligand L, Portugal FR, Roques BB, Santschi EM, Wilson KE, Toutain PL.
Pharmacokinetic-pharmacodynamic cutoff values for benzylpenicillin in horses to support the establishment of clinical breakpoints for benzylpenicillin antimicrobial susceptibility testing in horses.
Front Microbiol, 2023, 14, 1282949.

Ishii H, Shibuya M, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN.
First evidence of the incorporation of daprodustat and other hypoxia-inducible factor stabilizers into equine hair by passive transfer based on segmental quantitative analysis.
Journal of Pharmaceutical and Biomedical Analysis, 2023, 235, 115600.

Ishii H, Shibuya M, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN.
Segmental analysis and long-term monitoring of vadadustat in equine hair for the purpose of doping control.
Journal of Analytical Toxicology, 2023, 47(7), 623-631.

Yoshihara T, Tozaki T, Kawate K, Furukawa R, Kikuchi M, Ishige T, Kakoi H, Takasu M, Hobo S.
SNP array-based genomic inbreeding and relatedness analyses in an endangered Japanese equine breed, the Taishu horse.
Livest Sci. 2026;304:105898.

Furukawa R, Shinoda H, Tozaki T, Makino A, Yoshimura M, Iida T, Kawate K, Kikuchi M, Ishige T, Takahashi Y, Kato T, Kakoi H, Fukui E, Watanabe R.
Direct single-molecule detection of mRNA–LNP drugs in blood.
Anal Chem. 2026;98(1):756–765.

Cullen JN, Cieslak J, Petersen JL, Bellone RR, Finno CJ, Kalbfleisch TS, Calloe K, Capomaccio S, Cappelli K, Coleman SJ, Distl O, Durward-Akhurst SA, Giulotto E, Hamilton NA, Hill EW, Katz LM, Klaerke DA, Lindgren G, MacHugh DE, Mackowski M, MacLeod JN, Metzger J, Murphy BA, Orlando L, Raudsepp T, Silvestrelli M, Strand E, Tozaki T, Trachsel DS, Valderrama Figueroa LS, Velie BD, Wade CM, Waud B, Mickelson JR, McCue ME.
Charting the equine miRNA landscape: An integrated pipeline and browser for annotating, quantifying, and visualizing expression
PLoS Genet, 2025, 21(9), e1011835.

Furukawa R, Tozaki T, Mizukami K, Iwasaki Y, Kawate K, Kikuchi M, Ishige T, Momozawa Y, Fukui E, Kakoi H.
Mutation rate and spectrum of germline de novo mutations in a closed population of Thoroughbred horses
J Equine Vet Sci, 2025, 154, 105682.

Furukawa R, Tozaki T, Kawate K, Kikuchi M, Ishige T, Takahashi Y, Fukui E, Kakoi H.
Detection using chamber digital PCR with a DNA extraction-free method for gene-doping control.
Forensic Sci Int, 2025, 374, 112545.

Furukawa R, Tozaki T, Kikuchi M, Ishige T, Takahashi Y, Fukui E, Kakoi H.
A method for detecting gene doping in horse sports without DNA extraction.
Drug Test Anal, 2025, 17(3), 427-435.

Hisaeda K, Ono T, Shimokawa-Miyama T, Hata A, Iwata E, Hiasa Y, Ohzawa E, Tozaki T, Murase H, Takasu M, Nishii N, Kitagawa H.
Differences in serum iron concentrations between the summer and winter in Noma horses.
J Equine Sci, 2025, 36(1), 1-9.

Yokomori T, Tozaki T, Segawa T, Itou T.
Genomic regions and candidate genes associated with forehead whorl positioning in Thoroughbred horses.
J Equine Sci, 2025, 36(1), 11-18.

Yokomori T, Tozaki T, Ohnuma A, Ishimaru M, Sato F, Hori Y, Segawa T, Itou T.
Non-Synonymous Substitutions in Cadherin 13, Solute Carrier Family 6 Member 4, and Monoamine Oxidase A Genes are Associated with Personality Traits in Thoroughbred Horses.
Behav Genet, 2024, 54(4), 333-341.

Kikuchi M, Murase H, Urata K, Ishige T, Nagata SI, Tozaki T, Kakoi H, Ishiguro-Oonuma T, Kizaki K.
Evaluation of circulating miRNAs in mares approaching parturition.
Domest Anim Endocrinol, 2024, 89, 106879.

Rubin CJ, Hodge M, Naboulsi R, Beckman M, Bellone RR, Kallenberg A, J'Usrey S, Ohmura H, Seki K, Furukawa R, Ohnuma A, Davis BW, Tozaki T, Lindgren G, Andersson L.
An intronic copy number variation in Syntaxin 17 determines speed of greying and melanoma incidence in Grey horses.
Nat Commun, 2024,15(1), 7510.

Yoshihara T, Tozaki T, Nakaya S, Takasu M, Kawate K, Furukawa R, Kikuchi M, Isihge T, Nagata SI, Kakoi H, Hobo S.
Genetic characterization of phenotypic traits in endangered Taishu horse breed and their breeding strategy.
J Equine Vet Sci, 2024, 144, 105233.

Yokomori T, Ohnuma A, Tozaki T, Segawa T, Itou T.
Identification of personality-related candidate genes in Thoroughbred racehorses using a bioinformatics-based approach involving functionally annotated human genes.
Animals (Basel), 2023, 13(4), 769.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Short insertion and deletion discoveries via whole-genome sequencing of 101 Thoroughbred racehorses.
Genes (Basel), 2023, 14(3), 638.

Kikuchi M, Nagata SI, Ishige T, Minamijima Y, Hirota KI, Tozaki T, Kakoi H, Kizaki K.
Evaluation of the effect of glucocorticoid treatment on adrenocortical functions by monitoring endogenous hydrocortisone in horses.
J Vet Med Sci, 2023, 85(6), 647-652.

Masuda M, Nikadori A, Nikadori E, Uchida N, Takizawa Y, Ishimaru M, Yamamoto T, Yuasa R, Kugai H, Nagahama Y, Takasu M, Tozaki T.
Evaluation of genetic diversity using 31 microsatellites in Miyako horses.
J Equine Sci, 2023, 34(1), 1-6.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirora KI, Nagata SI.
Use of whole-genome variants and their frequency data to estimate haplotype structure in the Thoroughbred genome.
Anim Genet, 2023, 54(5), 662-663.

Ohnuma A, Tozaki T, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Multiplex detection of transgenes using πcode technology for gene doping control.
Anal Chem, 2023, 95(27), 10149-10154.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests.
J Equine Sci, 2023, 34(2), 21-27.

Ishige T, Kikuchi M, Kakoi H, Hirota KI, Ohnuma A, Tozaki T, Hirosawa Y, Tanaka S, Nagata SI.
Evaluation of parentage testing using single nucleotide polymorphism markers for draft horses in Japan.
Anim Sci J, 2023, 94(1), e13854.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Nagata SI.
Construction of an individual identification panel for horses using insertion and deletion markers
J Equine Sci, 2023, 34(3), 83-92.

Kikuchi M, Ishige T, Minamijima Y, Hirota KI, Nagata SI, Tozaki T, Kakoi H, Ishiguro-Oonuma T, Kizaki K.
Identification of potential miRNA biomarkers to detect hydrocortisone administration in horses.
Int J Mol Sci, 2023, 24(19), 14515.

Kobayashi I, Nakamura K, Saito I, Akita M, Tozaki T, Miyazaki M, Hano K, Takasu M.
Changes in population structure and genetic diversity of Misaki horses between 2015 and 2020.
J Vet Med Sci, 2023, 85(12), 1327-1329.

Amano T, Yokawa H, Masuda Y, Tozaki T, Kawai M, Shirai K.
Genome-wide search reveals the uniqueness of DNA regions associated with coat color and innate immunity in Hokkaido Native Horse.
Anim Sci J, 2023, 94(1), e13884.

Saito I, Nakamura K, Tozaki T, Hano K, Takasu M.
Genetic characterization of Japanese native horse breeds by genotyping variants that are associated with phenotypic traits.
J Equine Sci, 2023, 34(4), 115-120.

Kakoi H, Kikuchi M, Ishige T, Hirosawa Y, Tanaka S, Nagata SI.
Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses.
J Equine Sci, 2023, 34(2), 47-49.