Recent Publications
Research activities and resources accumulated from them empower LRC to enhance the testing services’ capabilities and qualities.
Drug Analysis Department
2022
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Ishii H, Shibuya M, So YM, Wong JKY, Ho ENM, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN
Long-term monitoring of IOX4 in horse hair and its longitudinal distribution with segmental analysis using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometry for the purpose of doping control
Drug Test Anal, 2022, in publishingREAD >>
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Ishii H, Leung GN, Yamashita S, Nagata SI, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I
Identification of potential biomarkers in urine and plasma after consumption of tobacco product in horses
Drug Test Anal, 2022, in publishingREAD >>
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Ishii H, Leung GN, Yamashita S, Nagata SI, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I
Comprehensive metabolic study of nicotine in equine plasma and urine using liquid chromatography/high-resolution mass spectrometry for the identification of unique biomarkers for doping control
J Chromatogr B Analyt Technol Biomed Life Sci, 1190, 123100, 2022READ >>
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Ishii H, Shibuya M, So YM, Wong JKY, Ho ENM, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN
Comprehensive metabolic study of IOX4 in equine urine and plasma using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometer for the purpose of doping control
Drug Test Anal, 14(2), 233-251, 2022READ >>
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Minamijima Y, Tozaki T, Kuroda T, Urayama S, Nomura M, Yamamoto K.
A comprehensive and comparative proteomic analysis of horse serum proteins in colitis.
Equine Vet J, 2022, in publishingREAD >>
Genetic Analysis Department
2025
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Furukawa R, Tozaki T, Kikuchi M, Ishige T, Takahashi Y, Fukui E, Kakoi H.
A method for detecting gene doping in horse sports without DNA extraction.
Drug Test Anal, 2025, 17(3), 427-435.READ >>
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Hisaeda K, Ono T, Shimokawa-Miyama T, Hata A, Iwata E, Hiasa Y, Ohzawa E, Tozaki T, Murase H, Takasu M, Nishii N, Kitagawa H.
Differences in serum iron concentrations between the summer and winter in Noma horses.
J Equine Sci, 2025, 36(1), 1-9.READ >>
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Yokomori T, Tozaki T, Segawa T, Itou T.
Genomic regions and candidate genes associated with forehead whorl positioning in Thoroughbred horses.
J Equine Sci, 2025, 36(1), 11-18.READ >>
2024
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Yokomori T, Tozaki T, Ohnuma A, Ishimaru M, Sato F, Hori Y, Segawa T, Itou T.
Non-Synonymous Substitutions in Cadherin 13, Solute Carrier Family 6 Member 4, and Monoamine Oxidase A Genes are Associated with Personality Traits in Thoroughbred Horses.
Behav Genet, 2024, 54(4), 333-341.READ >>
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Kikuchi M, Murase H, Urata K, Ishige T, Nagata SI, Tozaki T, Kakoi H, Ishiguro-Oonuma T, Kizaki K.
Evaluation of circulating miRNAs in mares approaching parturition.
Domest Anim Endocrinol, 2024, 89, 106879.READ >>
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Rubin CJ, Hodge M, Naboulsi R, Beckman M, Bellone RR, Kallenberg A, J'Usrey S, Ohmura H, Seki K, Furukawa R, Ohnuma A, Davis BW, Tozaki T, Lindgren G, Andersson L.
An intronic copy number variation in Syntaxin 17 determines speed of greying and melanoma incidence in Grey horses.
Nat Commun, 2024,15(1), 7510.READ >>
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Yoshihara T, Tozaki T, Nakaya S, Takasu M, Kawate K, Furukawa R, Kikuchi M, Isihge T, Nagata SI, Kakoi H, Hobo S.
Genetic characterization of phenotypic traits in endangered Taishu horse breed and their breeding strategy.
J Equine Vet Sci, 2024, 144, 105233.READ >>
2023
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Yokomori T, Ohnuma A, Tozaki T, Segawa T, Itou T.
Identification of personality-related candidate genes in Thoroughbred racehorses using a bioinformatics-based approach involving functionally annotated human genes.
Animals (Basel), 2023, 13(4), 769.READ >>
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Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Short insertion and deletion discoveries via whole-genome sequencing of 101 Thoroughbred racehorses.
Genes (Basel), 2023, 14(3), 638.READ >>
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Kikuchi M, Nagata SI, Ishige T, Minamijima Y, Hirota KI, Tozaki T, Kakoi H, Kizaki K.
Evaluation of the effect of glucocorticoid treatment on adrenocortical functions by monitoring endogenous hydrocortisone in horses.
J Vet Med Sci, 2023, 85(6), 647-652.READ >>
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Masuda M, Nikadori A, Nikadori E, Uchida N, Takizawa Y, Ishimaru M, Yamamoto T, Evaluation of genetic diversity using 31 microsatellites in Miyako horses.
Yuasa R, Kugai H, Nagahama Y, Takasu M, Tozaki T.
J Equine Sci, 2023, 34(1), 1-6.READ >>
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Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirora KI, Nagata SI.
Use of whole-genome variants and their frequency data to estimate haplotype structure in the Thoroughbred genome.
Anim Genet, 2023, 54(5), 662-663.READ >>
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Ohnuma A, Tozaki T, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Multiplex detection of transgenes using πcode technology for gene doping control.
Anal Chem, 2023, 95(27), 10149-10154.READ >>
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Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests.
J Equine Sci, 2023, 34(2), 21-27.READ >>
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Ishige T, Kikuchi M, Kakoi H, Hirota KI, Ohnuma A, Tozaki T, Hirosawa Y, Tanaka S, Nagata SI.
Evaluation of parentage testing using single nucleotide polymorphism markers for draft horses in Japan.
Anim Sci J, 2023, 94(1), e13854.READ >>
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Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Nagata SI.
Construction of an individual identification panel for horses using insertion and deletion markers
J Equine Sci, 2023, 34(3), 83-92.READ >>
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Kikuchi M, Ishige T, Minamijima Y, Hirota KI, Nagata SI, Tozaki T, Kakoi H, Ishiguro-Oonuma T, Kizaki K.
Identification of potential miRNA biomarkers to detect hydrocortisone administration in horses.
Int J Mol Sci, 2023, 24(19), 14515.READ >>
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Kobayashi I, Nakamura K, Saito I, Akita M, Tozaki T, Miyazaki M, Hano K, Takasu M.
Changes in population structure and genetic diversity of Misaki horses between 2015 and 2020.
J Vet Med Sci, 2023, 85(12), 1327-1329.READ >>
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Amano T, Yokawa H, Masuda Y, Tozaki T, Kawai M, Shirai K.
Genome-wide search reveals the uniqueness of DNA regions associated with coat color and innate immunity in Hokkaido Native Horse.
Anim Sci J, 2023, 94(1), e13884.READ >>
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Saito I, Nakamura K, Tozaki T, Hano K, Takasu M.
Genetic characterization of Japanese native horse breeds by genotyping variants that are associated with phenotypic traits.
J Equine Sci, 2023, 34(4), 115-120.READ >>
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Kakoi H, Kikuchi M, Ishige T, Hirosawa Y, Tanaka S, Nagata SI.
Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses.
J Equine Sci, 2023, 34(2), 47-49.READ >>
2022
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Maniego J, Pesko B, Habershon-Butcher J, Hincks P, Taylor P, Tozaki T, Ohnuma A, Stewart G, Proudman C, Ryder E.
Use of mitochondrial sequencing to detect gene doping in horses via gene editing and somatic cell nuclear transfer.
Drug Test Anal, 2022, 14(8), 1429-1437.READ >>
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Tozaki T, Ohnuma A, Nakamura K, Hano K, Takasu M, Takahashi Y, Tamura N, Sato F, Shimizu K, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Hamilton NA, Nagata SI.
Detection of indiscriminate genetic manipulation in Thoroughbred racehorses by targeted resequencing for gene-doping control.
Genes (Basel), 2022, 13(9), 1589.READ >>
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Yoshihara T, Kikuchi M, Akiba Y, Horiguchi M, Takasu M, Tozaki T.
Genetic diversity analysis and parentage verification of Taishu horses using 31 microsatellites.
J Equine Sci, 2022, 33(4), 63-69.READ >>
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Tozaki T, Hamilton NA.
Control of gene doping in human and horse sports.
Gene Ther, 29(3-4), 107-112, 2022READ >>
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Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Identification of processed pseudogenes in the genome of Thoroughbred horses: Possibility of gene-doping detection considering the presence of pseudogenes.
Anim Genet, 53(2), 183-192, 2022READ >>
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Tozaki T, Ohnuma A, Hamilton NA, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Low-copy transgene detection using nested digital polymerase chain reaction for gene-doping control.
Drug Test Anal, 14(2), 382-387, 2022READ >>
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Tozaki T, Kwak HG, Nakamura K, Takasu M, Ishii H, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Hirata M, Nirasawa T, Nagata SI.
Sequence determination of phosphorothioated oligonucleotides using MALDI-TOF mass spectrometry for controlling gene doping in equestrian sports.
Drug Test Anal, 14(1), 175-180, 2022READ >>
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Kinoshita Y, Kakoi H, Ishige T, Yamanaka T, Niwa H, Uchida-Fujii E, Nukada T, Ueno T.
Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.
J Vet Med Sci, 84(1), 129-132, 2022.READ >>