Recent Publications

Ishii H, Shibuya M, So YM, Wong JKY, Ho ENM, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN
Long-term monitoring of IOX4 in horse hair and its longitudinal distribution with segmental analysis using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometry for the purpose of doping control
Drug Test Anal, 2022, in publishing

Ishii H, Leung GN, Yamashita S, Nagata SI, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I
Identification of potential biomarkers in urine and plasma after consumption of tobacco product in horses
Drug Test Anal, 2022, in publishing

Ishii H, Leung GN, Yamashita S, Nagata SI, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I
Comprehensive metabolic study of nicotine in equine plasma and urine using liquid chromatography/high-resolution mass spectrometry for the identification of unique biomarkers for doping control
J Chromatogr B Analyt Technol Biomed Life Sci, 1190, 123100, 2022

Ishii H, Shibuya M, So YM, Wong JKY, Ho ENM, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN
Comprehensive metabolic study of IOX4 in equine urine and plasma using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometer for the purpose of doping control
Drug Test Anal, 14(2), 233-251, 2022

Minamijima Y, Tozaki T, Kuroda T, Urayama S, Nomura M, Yamamoto K.
A comprehensive and comparative proteomic analysis of horse serum proteins in colitis.
Equine Vet J, 2022, in publishing

Kuroda T, Minamijima Y, Niwa H, Tamura N, Mita H, Fukuda K, Kaimachi M, Suzuki Y, Enoki Y, Taguchi K, Matsumoto K, Toutain PL, Bousquet-Melou A, Kasashima Y
Rational dosage regimens for cephalothin and cefazolin using pharmacokinetics and pharmacodynamics analysis in healthy horses
Equine Vet J, 53(6), 1239-1249, 2021

Kuroda T, Minamijima Y, Nomura M, Yamashita S, Yamada M, Nagata S, Mita H, Tamura N, Fukuda K, Kuwano A, Kusano K, Toutain PL, Sato F.
Medication control of flunixin in racing horses: Possible detection times using Monte Carlo simulations.
Equine Vet J, 00, 1-10, 2021

Ishii H, Shibuya M, Leung GN, Nozawa S, Yamashita S, Yamada M, Kushiro A, Kasashima Y, Okada J, Kawasaki K, Kijima-Suda I
Detection and longitudinal distribution of GW1516 and its metabolites in equine hair for doping control using liquid chromatography/high-resolution mass spectrometry
Rapid Commun Mass Spectrom, 35(8), e9050, 2021

Ohnuma K, Uchida T, Leung GN, Ueda T, Obara T, Ishii H
Establishment of a post-race biomarkers database and application of pathway analysis to identify potential biomarkers in post-race equine plasma
Drug Test Anal, 1-14, 2021

Ishii H, Shibuya M, Leung GN, Yamashita S, Yamada M, Kushiro A, Kasashima Y, Okada J, Kawasaki K, Kijima-Suda I
Metabolic study of GW1516 in equine urine using liquid chromatography/electrospray ionization Q-Exactive high-resolution mass spectrometry for doping control
Rapid Commun Mass Spectrom, 35(5), e9028, 2021

Minamijima Y, Niwa H, Uchida E, Yamamoto K.
Comparison of the proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratory disease associated with transport using mass spectrometry-based proteomics.
J Equine Sci, 32(1), 11-15, 2021

Ishii H, Leung GN, Yamashita S, Yamada M, Kushiro A, Kasashima Y, Okada J, Kawasaki K and Kijima-Suda I
Doping control analysis of GW1516 in equine plasma using liquid chromatography/electrospray ionization Q-Exactive high-resolution mass spectrometry
Rapid Commun Mass Spectrom, 34(23), e8920, 2020

Ishii H, Obara T, Kusano K and Kijima-Suda I
Quantitative analysis of paracetamol, metacetamol, and orthocetamol in equine urine from racehorses in Japan using liquid chromatography-electrospray ionization-tandem mass spectrometry
Drug Test Anal, 12(8), 1196-1202, 2020

Ishii H, Obara T and Kijima-Suda I
Investigation of plasma concentrations of paracetamol, metacetamol, and orthocetamol in Japanese racehorses using liquid chromatography-electrospray ionisation-tandem mass spectrometry
Drug Test Anal, 12(7), 929-937, 2020

Mizobe F, Mori M, Nagata SI, Yamashita S, Okada J and Kusano K
Presence of Antimicrobials in Postrace Samples in Japanese Thoroughbred Racing
J Equine Vet Sci, 91, 103115, 2020

Tozaki T, Hamilton NA.
Control of gene doping in human and horse sports.
Gene Ther, 29(3-4), 107-112, 2022

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Identification of processed pseudogenes in the genome of Thoroughbred horses: Possibility of gene-doping detection considering the presence of pseudogenes.
Anim Genet, 53(2), 183-192, 2022

Tozaki T, Ohnuma A, Hamilton NA, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Low-copy transgene detection using nested digital polymerase chain reaction for gene-doping control.
Drug Test Anal, 14(2), 382-387, 2022

Tozaki T, Kwak HG, Nakamura K, Takasu M, Ishii H, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Hirata M, Nirasawa T, Nagata SI.
Sequence determination of phosphorothioated oligonucleotides using MALDI-TOF mass spectrometry for controlling gene doping in equestrian sports.
Drug Test Anal, 14(1), 175-180, 2022

Kinoshita Y, Kakoi H, Ishige T, Yamanaka T, Niwa H, Uchida-Fujii E, Nukada T, Ueno T.
Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.
J Vet Med Sci, 84(1), 129-132, 2022.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests.
J Equine Sci, 32(4), 125-134, 2021

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota K, Kusano K, Nagata S.
Simulated validation of intron-less transgene detection using DELLY for gene-doping control in horse sports.
Anim Genet, 52(5), 759-761, 2021

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Robustness of digital PCR and real-time PCR against inhibitors in transgene detection for gene doping control in equestrian sports.
Drug Test Anal, 13(10), 1768-1775, 2021

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Rare and common variant discovery by whole-genome sequencing of 101 Thoroughbred racehorses.
Sci Rep, 11(1), 16057, 2021

Tozaki T, Ohnuma A, Iwai S, Kikuchi M, Ishige T, Kakoi H, Hirota K, Kusano K, Nagata S.
Robustness of Digital PCR and Real-Time PCR in Transgene Detection for Gene-Doping Control.
Anal Chem, 93(18), 7133-7139, 2021

Tozaki T, Ohnuma A, Takasu M, Nakamura K, Kikuchi M, Ishige T, Kakoi H, Hirora KI, Tamura N, Kusano K, Nagata SI.
Detection of non-targeted transgenes by whole-genome resequencing for gene-doping control.
Gene Ther, 28(3-4), 199-205, 2021

Hatai H, Hatazoe T, Seo H, Tozaki T, Ishikawa S, Miyoshi N, Misumi K, Hobo S.
Primary sinonasal malignant melanoma with systemic metastasis in a non-gray horse.
J Vet Diagn Invest, 33(2), 379-383, 2021

Kakoi H, Kikuchi M, Ishige T, Nagata S, Hirosawa Y, Tanaka S, Kishinami T.
Investigation of erythrocyte antigen frequencies in draft horse populations in Japan to assess blood donor suitability.
J Equine Sci, 32(1), 17-19, 2021.

Kuroda T, Minamijima Y, Nomura M, Yamashita S, Yamada M, Nagata S, Mita H, Tamura N, Fukuda K, Kuwano A, Kusano K, Toutain PL, Sato F.
Medication control of flunixin in racing horses: Possible detection times using Monte Carlo simulations
Equine Vet J, doi: 10.1111/evj.13532. 2021.

Nakamura M, Tozaki T, Kakoi H, Nakamura K, Rajabi-Toustani R, Ohba Y, Matsubara T, Takasu M.
Decreased genetic diversity in Kiso horses revealed through annual microsatellite genotyping.
J Vet Med Sci, 82(4), 503-540, 2020

Murase H, Ochi A, Tozaki T, Kakoi H, Munkhtuul T, Kurimoto S, Sato F, Hada T.
A case of equine cryptorchidism with undetectable serum anti-Müllerian hormone.
J Vet Med Sci, 82(2), 209-211, 2020

McGivney BA, Han H, Corduff LR, Katz LM, Tozaki T, MacHugh DE, Hill EW.
Genomic inbreeding trends, influential sire lines and selection in the global Thoroughbred horse population.
Sci Rep, 10(1), 466, 2020

Amano T, Tozaki T, Takasu M, Onogi A, Yamada F, Kawai M, Ueda J.
Changes of sires in a breeding farm enables maintenance of DNA-level genetic variation in a produced herd of Hokkaido Native Horses.
Anim Sci J, 91(1), e13318, 2020

Tamura N, Okano A, Kuroda T, Niwa H, Kusano K, Matsuda Y, Fukuda K, Mita H, Nagata S.
Utility of systemic voriconazole in equine keratomycosis based on pharmacokinetic-pharmacodynamic analysis of tear fluid following oral administration.
Vet Ophthalmol, 23(4):640-647, 2020

Kuroda T, Nagata S, Tamura N, Kinoshita Y, Niwa H, Mita H, Minami T, Fukuda K, Hobo S, Kuwano A.
Single-dose pharmacokinetics of orally administered metronidazole and intravenously administered imipenem in healthy horses and computer-based simulation of pleural fluid concentrations with multiple dosing.
Am J Vet Res, 81(10):783-789.2020

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Hamilton NA, Kusano K, Nagata SI.
Whole-genome resequencing using genomic DNA extracted from horsehair roots for gene-doping control in horse sports.
J Equine Sci, 31(4), 75-83, 2020

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Microfluidic Quantitative PCR Detection of 12 Transgenes from Horse Plasma for Gene Doping Control.
Genes (Basel), 11(4), 457, 2020

Tozaki T, Kusano K, Ishikawa Y, Kushiro A, Nomura M, Kikuchi M, Kakoi H, Hirota K, Miyake T, Hill EW, Nagata S.
A candidate-SNP retrospective cohort study for fracture risk in Japanese Thoroughbred racehorses.
Anim Genet, 51(1), 43-50, 2020