Recent Publications

Nomura M, Kuroda T, Ohta M, Kusano K, Minamijima Y, Nagata S.
Pharmacokinetics of Salbutamol in Thoroughbred Horses After a Single Intravenous or Inhaled Administration.
J Vet Pharmacol Ther, 2025, 48(2), 76-85.

Shimizu Y, Sugai-Bannai M, Saito K, Hirano-Kodaira M, Leung GN.
Identification and differentiation of somapacitan, a long-acting growth hormone derivative, and recombinant human growth hormone in equine plasma by LC-HRMS for doping control purpose.
Growth Hormone & IGF Research, 2025, 80, 101628.

Kuroda T, Minamijima Y, Niwa H, Mita H, Tamura N, Fukuda K, Toutain PL, Ohta M.
Pharmacokinetics/pharmacodynamics cut-off determination for fosfomycin using Monte Carlo simulation in healthy horses.
J Vet Med Sci, 2024, 86(4), 413-420.

Mita H, Kuroda T, Minamijima Y, Tamura N, Ohta M.
Concentration of Marbofloxacin in equine subcutaneous tissue fluid after subcutaneous administration in encapsulated microparticles.
J Equine Vet Sci, 2024, 141, 105148.

Kuroda T, Minamijima Y, Kinman CK, Takahashi Y, Ebisuda Y, Inoue K, Ishikawa H, Mita H, Tamura N, Nukada T, Toutain PL, Ohta M.
Rational quinidine dosage regimen for atrial fibrillation in Thoroughbred racehorses based on population pharmacokinetics.
Front Vet Sci, 2024, 11, 1454342.

Uchida T, Kisugi T, Ishii H, Yamada M, Kinoshita K, Leung GN.
Broad-spectrum and sensitive screening of more than 1000 compounds in equine urine using liquid chromatography/highresolution mass spectrometry
Rapid Commun Mass Spectrom, 2024, 38, e9856.

Ohnuma K, Hirano-Kodaira M, Bannai M, Shimizu Y, Yamada M, Kinoshita K, Leung GN, Ishii H.
A Broad-Spectrum Peptide Screening Method Using an Optimized Solid-Phase Extraction and Liquid Chromatography–High-Field Asymmetric Ion Mobility Spectrometry–Mass Spectrometry for Doping Control in Equine Urine.
Anal Methods, 2024, 16, 8250-8267.

Ishii H, Shigematsu R, Takemoto S, Ishikawa Y, Mizobe F, Nomura M, Arima D, Kunii H, Yuasa R, Yamanaka T, Tanabe S, Nagata S, Yamada M, Leung GN.
Quantification of osilodrostat in horse urine using LC/ESI-HRMS to establish an elimination profile for doping control.
Bioanalysis, 2024, 16(17-18), 947-958.

Ishii H, Shigematsu R, Takemoto S, Ishikawa Y, Mizobe F, Nomura M, Arima D, Kunii H, Yuasa R, Yamanaka T, Tanabe S, Nagata S, Yamada M, Leung GN.
Metabolic Pathway of Osilodrostat in Equine Urine Established through High-resolution Mass Spectrometric Characterization for Doping Control.
Current Drug Metabolism, 2024, 25(7), 489-504.

Minamijima Y, Kuroda T, Kamiya T, Sone Y, Wakuno A, Ito H, Nomura M, Leung GN, Kinoshita K, Yamada M.
Evaluation of plasma and urine pharmacokinetics of tranexamic acid for equine medication control.
J Vet Pharmacol Ther, 2024, 47(1), 21-27.

Kuroda T, Minamijima Y, Niwa H, Mita H, Tamura N, Fukuda K, Ohta M.
Pharmacokinetic/pharmacodynamic analysis of cephalothin after intramuscular administration in Thoroughbred horses.
Journal of Equine Science, 2023, 34(4), 111-114.

Lallemand EA, Bousquet-Mélou A, Chapuis L, Davis J, Ferran AA, Kukanich B, Kuroda T, Lacroix MZ, Minamijima Y, Olsén L, Pelligand L, Portugal FR, Roques BB, Santschi EM, Wilson KE, Toutain PL.
Pharmacokinetic-pharmacodynamic cutoff values for benzylpenicillin in horses to support the establishment of clinical breakpoints for benzylpenicillin antimicrobial susceptibility testing in horses.
Front Microbiol, 2023, 14, 1282949.

Ishii H, Shibuya M, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN.
First evidence of the incorporation of daprodustat and other hypoxia-inducible factor stabilizers into equine hair by passive transfer based on segmental quantitative analysis.
Journal of Pharmaceutical and Biomedical Analysis, 2023, 235, 115600.

Ishii H, Shibuya M, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN.
Segmental analysis and long-term monitoring of vadadustat in equine hair for the purpose of doping control.
Journal of Analytical Toxicology, 2023, 47(7), 623-631.

Minamijima Y, Tozaki T, Kuroda T, Urayama S, Nomura M, Yamamoto K.
A comprehensive and comparative proteomic analysis of horse serum proteins in colitis.
Equine Vet J, 2022, 54(6), 1039-1049.

Ishii H, Shibuya M, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Sato F, Kuroda T, Yamada M, Leung GN.
Generic approach for the discovery of drug metabolites in horses based on data-dependent acquisition by liquid chromatography high-resolution mass spectrometry and its applications to pharmacokinetic study of daprodustat.
Anal Bioanal Chem, 2022, 414(28), 8125-8142.

Ishii H, Shibuya M, Leung GN, Yamashita S, Nagata S, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I.
Additional studies on nicotine exposure in horses: Accurate quantification and elimination profiles of potential biomarkers in plasma and urine.
Rapid Commun Mass Spectrom, 2022, 36(23), e9396.

Ishii H, Shibuya M, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Sato F, Kuroda T, Yamada M, Leung GN.
Pharmacokinetic Study of Vadadustat and High-Resolution Mass Spectrometric Characterization of its Novel Metabolites in Equines for the Purpose of Doping Control.
Curr Drug Metab, 2022, 23(10), 850-865.

Ishii H, Leung GN, Yamashita S, Nagata SI, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I.
Comprehensive metabolic study of nicotine in equine plasma and urine using liquid chromatography/high-resolution mass spectrometry for the identification of unique biomarkers for doping control.
J Chromatogr B Analyt Technol Biomed Life Sci, 2022, 1190, 123100.

Ishii H, Leung GN, Yamashita S, Nagata SI, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I.
Identification of potential biomarkers in urine and plasma after consumption of tobacco product in horses.
Drug Test Anal, 2022, 14(5), 902-914.

Ishii H, Shibuya M, So YM, Wong JKY, Ho ENM, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN.
Long-term monitoring of IOX4 in horse hair and its longitudinal distribution with segmental analysis using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometry for the purpose of doping control.
Drug Test Anal, 2022, 14(7), 1244-1254.

Ishii H, Shibuya M, So YM, Wong JKY, Ho ENM, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN.
Comprehensive metabolic study of IOX4 in equine urine and plasma using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometer for the purpose of doping control.
Drug Test Anal, 2022, 14(2), 233-251.

Ohnuma K, Uchida T, Leung GN, Ueda T, Obara T, Ishii H.
Establishment of a post-race biomarkers database and application of pathway analysis to identify potential biomarkers in post-race equine plasma.
Drug Test Anal, 2022, 14(5), 915-928.

Ishii H, Shibuya M, So YM, Wong JKY, Ho ENM, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN
Long-term monitoring of IOX4 in horse hair and its longitudinal distribution with segmental analysis using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometry for the purpose of doping control
Drug Test Anal, 2022, in publishing

Ishii H, Leung GN, Yamashita S, Nagata SI, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I
Identification of potential biomarkers in urine and plasma after consumption of tobacco product in horses
Drug Test Anal, 2022, in publishing

Ishii H, Leung GN, Yamashita S, Nagata SI, Kushiro A, Sakai S, Toju K, Okada J, Kawasaki K, Kusano K, Kijima-Suda I
Comprehensive metabolic study of nicotine in equine plasma and urine using liquid chromatography/high-resolution mass spectrometry for the identification of unique biomarkers for doping control
J Chromatogr B Analyt Technol Biomed Life Sci, 1190, 123100, 2022

Ishii H, Shibuya M, So YM, Wong JKY, Ho ENM, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN
Comprehensive metabolic study of IOX4 in equine urine and plasma using liquid chromatography/electrospray ionization Q Exactive high-resolution mass spectrometer for the purpose of doping control
Drug Test Anal, 14(2), 233-251, 2022

Minamijima Y, Tozaki T, Kuroda T, Urayama S, Nomura M, Yamamoto K.
A comprehensive and comparative proteomic analysis of horse serum proteins in colitis.
Equine Vet J, 2022, in publishing

Furukawa R, Tozaki T, Kawate K, Kikuchi M, Ishige T, Takahashi Y, Fukui E, Kakoi H.
Detection using chamber digital PCR with a DNA extraction-free method for gene-doping control.
Forensic Sci Int, 2025, 374, 112545.

Furukawa R, Tozaki T, Kikuchi M, Ishige T, Takahashi Y, Fukui E, Kakoi H.
A method for detecting gene doping in horse sports without DNA extraction.
Drug Test Anal, 2025, 17(3), 427-435.

Hisaeda K, Ono T, Shimokawa-Miyama T, Hata A, Iwata E, Hiasa Y, Ohzawa E, Tozaki T, Murase H, Takasu M, Nishii N, Kitagawa H.
Differences in serum iron concentrations between the summer and winter in Noma horses.
J Equine Sci, 2025, 36(1), 1-9.

Yokomori T, Tozaki T, Segawa T, Itou T.
Genomic regions and candidate genes associated with forehead whorl positioning in Thoroughbred horses.
J Equine Sci, 2025, 36(1), 11-18.

Yokomori T, Tozaki T, Ohnuma A, Ishimaru M, Sato F, Hori Y, Segawa T, Itou T.
Non-Synonymous Substitutions in Cadherin 13, Solute Carrier Family 6 Member 4, and Monoamine Oxidase A Genes are Associated with Personality Traits in Thoroughbred Horses.
Behav Genet, 2024, 54(4), 333-341.

Kikuchi M, Murase H, Urata K, Ishige T, Nagata SI, Tozaki T, Kakoi H, Ishiguro-Oonuma T, Kizaki K.
Evaluation of circulating miRNAs in mares approaching parturition.
Domest Anim Endocrinol, 2024, 89, 106879.

Rubin CJ, Hodge M, Naboulsi R, Beckman M, Bellone RR, Kallenberg A, J'Usrey S, Ohmura H, Seki K, Furukawa R, Ohnuma A, Davis BW, Tozaki T, Lindgren G, Andersson L.
An intronic copy number variation in Syntaxin 17 determines speed of greying and melanoma incidence in Grey horses.
Nat Commun, 2024,15(1), 7510.

Yoshihara T, Tozaki T, Nakaya S, Takasu M, Kawate K, Furukawa R, Kikuchi M, Isihge T, Nagata SI, Kakoi H, Hobo S.
Genetic characterization of phenotypic traits in endangered Taishu horse breed and their breeding strategy.
J Equine Vet Sci, 2024, 144, 105233.

Yokomori T, Ohnuma A, Tozaki T, Segawa T, Itou T.
Identification of personality-related candidate genes in Thoroughbred racehorses using a bioinformatics-based approach involving functionally annotated human genes.
Animals (Basel), 2023, 13(4), 769.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Short insertion and deletion discoveries via whole-genome sequencing of 101 Thoroughbred racehorses.
Genes (Basel), 2023, 14(3), 638.

Kikuchi M, Nagata SI, Ishige T, Minamijima Y, Hirota KI, Tozaki T, Kakoi H, Kizaki K.
Evaluation of the effect of glucocorticoid treatment on adrenocortical functions by monitoring endogenous hydrocortisone in horses.
J Vet Med Sci, 2023, 85(6), 647-652.

Masuda M, Nikadori A, Nikadori E, Uchida N, Takizawa Y, Ishimaru M, Yamamoto T, Evaluation of genetic diversity using 31 microsatellites in Miyako horses.
Yuasa R, Kugai H, Nagahama Y, Takasu M, Tozaki T.
J Equine Sci, 2023, 34(1), 1-6.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirora KI, Nagata SI.
Use of whole-genome variants and their frequency data to estimate haplotype structure in the Thoroughbred genome.
Anim Genet, 2023, 54(5), 662-663.

Ohnuma A, Tozaki T, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Multiplex detection of transgenes using πcode technology for gene doping control.
Anal Chem, 2023, 95(27), 10149-10154.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI.
Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests.
J Equine Sci, 2023, 34(2), 21-27.

Ishige T, Kikuchi M, Kakoi H, Hirota KI, Ohnuma A, Tozaki T, Hirosawa Y, Tanaka S, Nagata SI.
Evaluation of parentage testing using single nucleotide polymorphism markers for draft horses in Japan.
Anim Sci J, 2023, 94(1), e13854.

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Nagata SI.
Construction of an individual identification panel for horses using insertion and deletion markers
J Equine Sci, 2023, 34(3), 83-92.

Kikuchi M, Ishige T, Minamijima Y, Hirota KI, Nagata SI, Tozaki T, Kakoi H, Ishiguro-Oonuma T, Kizaki K.
Identification of potential miRNA biomarkers to detect hydrocortisone administration in horses.
Int J Mol Sci, 2023, 24(19), 14515.

Kobayashi I, Nakamura K, Saito I, Akita M, Tozaki T, Miyazaki M, Hano K, Takasu M.
Changes in population structure and genetic diversity of Misaki horses between 2015 and 2020.
J Vet Med Sci, 2023, 85(12), 1327-1329.

Amano T, Yokawa H, Masuda Y, Tozaki T, Kawai M, Shirai K.
Genome-wide search reveals the uniqueness of DNA regions associated with coat color and innate immunity in Hokkaido Native Horse.
Anim Sci J, 2023, 94(1), e13884.

Saito I, Nakamura K, Tozaki T, Hano K, Takasu M.
Genetic characterization of Japanese native horse breeds by genotyping variants that are associated with phenotypic traits.
J Equine Sci, 2023, 34(4), 115-120.

Kakoi H, Kikuchi M, Ishige T, Hirosawa Y, Tanaka S, Nagata SI.
Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses.
J Equine Sci, 2023, 34(2), 47-49.

Maniego J, Pesko B, Habershon-Butcher J, Hincks P, Taylor P, Tozaki T, Ohnuma A, Stewart G, Proudman C, Ryder E.
Use of mitochondrial sequencing to detect gene doping in horses via gene editing and somatic cell nuclear transfer.
Drug Test Anal, 2022, 14(8), 1429-1437.

Tozaki T, Ohnuma A, Nakamura K, Hano K, Takasu M, Takahashi Y, Tamura N, Sato F, Shimizu K, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Hamilton NA, Nagata SI.
Detection of indiscriminate genetic manipulation in Thoroughbred racehorses by targeted resequencing for gene-doping control.
Genes (Basel), 2022, 13(9), 1589.

Yoshihara T, Kikuchi M, Akiba Y, Horiguchi M, Takasu M, Tozaki T.
Genetic diversity analysis and parentage verification of Taishu horses using 31 microsatellites.
J Equine Sci, 2022, 33(4), 63-69.

Tozaki T, Hamilton NA.
Control of gene doping in human and horse sports.
Gene Ther, 29(3-4), 107-112, 2022

Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Identification of processed pseudogenes in the genome of Thoroughbred horses: Possibility of gene-doping detection considering the presence of pseudogenes.
Anim Genet, 53(2), 183-192, 2022

Tozaki T, Ohnuma A, Hamilton NA, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Low-copy transgene detection using nested digital polymerase chain reaction for gene-doping control.
Drug Test Anal, 14(2), 382-387, 2022

Tozaki T, Kwak HG, Nakamura K, Takasu M, Ishii H, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Hirata M, Nirasawa T, Nagata SI.
Sequence determination of phosphorothioated oligonucleotides using MALDI-TOF mass spectrometry for controlling gene doping in equestrian sports.
Drug Test Anal, 14(1), 175-180, 2022

Kinoshita Y, Kakoi H, Ishige T, Yamanaka T, Niwa H, Uchida-Fujii E, Nukada T, Ueno T.
Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.
J Vet Med Sci, 84(1), 129-132, 2022.